They arrived in the Stone Circle in Kings Meadow at 12pm on Friday June 22 to send a "message of peace to the world".
The previous record was set by a crowd of 5,814 at the Ithaca Festival in NY back in 2009.
According to NME they pulled in 15,000 revellers. "Peace and love comes about simply by giving peace and love".
Glastonbury Festival fell silent for a minute on Friday in memory of recent terror attacks in the United Kingdom and the devastating Grenfell Tower fire in London, before Hacienda Classical eased revellers into the first day of music at the world's biggest greenfield festival.
Just prior to the peace sign gathering, Glastonbury paid another tribute to the victims of the attacks. "We're always tweaking. It never stands still".
A group of participants that had attended the Ariana Grande concert at Manchester Arena - where the suicide bombing that killed 22 happened on May 22 - stressed that it is important for people to feel comfortable gathering in large crowds and attending events such as this one again.
Glastonbury festival opened with a minute's silence for those who lost their lives in the recent atrocities in Manchester and London.
Peter Hook, the bass player from Manchester bands Joy Division and New Order, led the crowd on the Pyramid Stage in reflecting on "our hopes and our prayers for life, love and freedom, the things we are here to celebrate".