Protesters from the Greek communist party attached ropes to the 3.5-metre bronze statue and were preparing to saw through the base of the structure..
On Monday, supporters of Greece's communist party attempted to tear down a statue of President Harry Truman in response to USA -led airstrikes in Syria, according to reports.
The statue suffered minor damage while at least three protesters were injured, with blood trickling from their heads.
Several hundred KKE supporters joined a march to the US Embassy, a couple of miles from the statue, in the third demonstration in Athens against Saturday's air strikes on what Western powers said were Syrian chemical weapons installations.
The statue was erected in 1963 in recognition of America's 33rd president who approved the 1948 Marshall Plan of economic assistance that helped rebuild war-shattered Greece.
"The communist and workers' parties express their internationalist solidarity to the people of Syria and the other peoples of the region, they call upon the working class, the people's forces to reinforce the struggle against the imperialist interventions and wars, of the North Atlantic Treaty Organisation, the U.S. and the European Union", the group said in a statement, which was signed by members of communist parties across Europe.
The protesters have also burnt the American flag.
The statue had to be removed and restored in 1986 after being bombed.
Many also believe Washington backed the military junta that ruled Greece from 1967-74 as well as the Turkish invasion of Cyprus in 1974 following a brief Greek-backed coup.