De Niro delivered his latest anti-Trump comments while introducing Bruce Springsteen to the well-heeled audience there to see Broadway's best recognised. "I watched him last night and truly believe me may be 'punch-drunk'".
Many on Twitter quickly pointed out that he confused "to" and "too" in his tweet.
Yesterday De Niro apologised - not for his comments but on behalf of the U.S. president.
In January he also hit out at Mr Trump at another award night.
"I'm going to say one thing", he said, raising his fists in the air.
REUTERSRobert De Niro hit out at the US president at the Tony Awards on Sunday
De Niro's first prominent act of speaking out against Trump arrived in a PSA video preceding the presidential election in October 2016, when the actor called Trump a "dog", a "mutt", a "con", and an "idiot", and said of then-candidate Trump, "I'd like to punch him in the face".
"It's no longer 'down with Trump, ' it's 'f-k Trump, ' " the 74-year-old performer - who won an Academy Award for playing a boxer in 1980's "Raging Bull" - said.
Rather than dwell on the momentous occasion as North Korea and the US finally sat down and pursued a diplomatic means to avert the possibility of World War III, President Trump has taken a few minutes out of his busy schedule to have a pop at an actor that appeared in The Adventures of Rocky & Bullwinkle. On Monday, the actor apologized to Canadians for the "idiotic behavior of my president".
"It's a disgrace. And I apologize to Justin Trudeau and the other people at the G7".
After a few tweets boasting about his historic summit with Kim Jong-un, the President reverted to name calling, criticizing the film star, whom he called "a very low IQ individual".