The highway is expected to remain closed Friday morning, Ventura County Fire Chief Mark Lorenzen said.
Evacuations in Hidden Hills and all portions in the City of Los Angeles have been revised from mandatory to voluntary, the Fire Department stated.
The LA County Sheriff's Office said it was receiving "many emergent phone calls" from people who "need our help to evacuate" and urged motorists in the area to be aware of emergency vehicles.
The erupted Thursday near the northwest corner of the city of Los Angeles as the region's notorious Santa Ana winds gusted, triggering overnight evacuations of an estimated 75,000 homes in western Los Angeles County and eastern Ventura County.
Cal Fire Capt. Scott McLean said a couple of thousand structures were destroyed in the town about 180 miles northeast of San Francisco.
The L.A. Times reported that a total of 75,000 homes in both Ventura and Los Angeles counties are now under mandatory or voluntary evacuation orders, including Westlake Village and parts of Calabasas, where numerous entertainers including Alyssa Milano and Kim Kardashian have lavish homes.
Malibu itself - a city of about 12,000 people and known for beachside homes owned by celebrities and millionaires - was put under an evacuation order Friday morning.
It has so far consumed between 8,000 to 10,000 acres and forced evacuations for Cal State Channel Islands and the Dos Vientos and Camarillo Springs areas.
Pepperdine on Friday closed its Malibu and Calabasas campuses because of the approaching blaze. Her deck provided a strong vantage point to monitor the spread of the fire. "We are still not out of the woods yet". "We have found a safe hotel ..."
"This is a very unsafe wind driven fire".
Where are the other fires?
Zoo staff have begun to evacuate some animals - starting with the lemurs - but the fire has not spread as fast as the Malibu blaze due to lighter winds.
Just 12 minutes after it started Thursday afternoon, the flames spread across USA 101, leaving several drivers temporarily stranded.
Residents posted on social media to share their views of the flames almost consuming the hillside in the Newbury Park area of western Thousand Oaks. The fire got started north of the 101 freeway in Simi Valley where it burned a number of homes last night.
Pepperdine University shut down its Malibu and Calabasas campuses and initiated shelter-in-place protocols as the fire roared southward, sweeping into the Santa Monica mountains.
Cal Fire Director Ken Pimlott said a "very significant, risky weather pattern" would continue through the end of the weekend.
"We expect to see another breezy offshore wind event develop on Sunday and into Monday, although it will likely be a notch or two weaker than the current event", reported the forecast team.