Ross, who performed some of her set while walking through the audience, was introduced onstage by her 9-year-old grandson, Raif-Henok Emmanuel Kendrick, son of her daughter Rhonda Ross Kendrick.
Get push notifications with news, features and more. "Together we have no limits", she said. "Young people like me can look up to her for her independence, confidence and willingness to be her unique self".
Diana also walked through the audience during her rendition of "Reach Out and Touch (Somebody's Hand)" and, in an impromptu moment, sang along with Will Smith's son Jaden. "So, ladies and gentlemen, please welcome my grand-mommy Diana Ross".
Ross' first solo Grammy nomination was for Best Contemporary Vocal Performance-Female for the hit "Ain't No Mountain High Enough" at the 13th Grammy Awards in 1970. The singer - who sang a medley of her hits in a tribute to herself, in honor of her upcoming 75th birthday - left her peers in awe.
The music icon then asked the audience to sing along with her.
After telling the crowd "don't be lazy" as she prompted everyone to stand, she ended the performance by exclaiming, "Happy birthday to me!"
The Grammys said on its Twitter feed, the "very special Grammys performance" was meant to honor Ross' "landmark career and contributions to music".